Induction of heat shock proteins DnaK, GroEL, and GroES by salt stress in Lactococcus lactis

Article Abstract:

Western blotting and direct N-terminal amino acid sequencing were utilized to determine the position of DnaK, GroEL and GroES in two-dimensional gels and the effects of salt or heat stress on heat shock protein expression. Exposure of Lactococcus lactis subsp. cremoris to salt stress induced the expression of DnaK, GroEL and GroES. Furthermore, the induction of the heat shock proteins was mediated by the accumulation of denatured proteins due to lowered water activity during the plasmolysis of the osmotically shocked Lactococcus lactis cells.

Lactobacillus, Microbial genetics

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Close correlation between heat shock response and cytotoxicity in Neurospora crassa treated with aliphatic alcohols and phenols

Article Abstract:

Addition of either alcohols or phenols stimulates the highest levels of heat shock proteins in Neurospora crassa, while they inhibit the overall protein synthesis. Lipophilicities of the alcohols account for different abilities of these alcohols to induce the heat shock response. Induction of heat shock protein occurs along with constrained cell metabolism. The heat shock response is a good probe to identify cell damage, but it is not a sensitive marker for toxicity tests.

Physiological aspects, Phenols, Phenols (Class of compounds), Alcohols

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Differential expression of glucose-regulated (grp78) and heat-shock-inducible (hsp70) genes during asexual development of Neurospora crassa

Article Abstract:

The vegetative life cycle of Neurospora crassa is studied to identify changes in the expression of glucose-regulated (grp78) and heat-shock-inducible (hsp70) genes. Results indicate high constitutive amounts of grp78 mRNA during exponential growth. Strongest induction of hsp70 is seen during the late phase of conidiation. A similar increase in both mRNA species is observed in surface mycelium.

Protein folding

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