Production of extracellular proteins by the biocontrol fungus Gliocladium virens
Article Abstract:
The production of extracellular proteins by Gliocladium virens grown in minimal carbon-containing medium is discussed. An eight-day monitoring period was conducted on a number of enzymatic activities in culturesof G. virens. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to separate the extracellular polypeptides produced by the fungus as the relative concentrations of some of these polypeptides were determined. Results indicate that a combination of gene regulation and protein degradation regulatethe extracellular proteins in cultures of G. virens.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
Partial purification and characterization of manganese-oxidizing factors of Pseudomonas fluorescens GB-1
Article Abstract:
A research was conducted to investigate the partial purification of Mn2+-oxidizing proteins isolated from cell extracts of Pseudomonas fluorescens GB-1 and the nature of the Mn2+-oxidizing activity. A chemical kit was utilized for the biochemical identification of bacteria while the Mn2+-oxidizing activity was determined colorimetrically with Leukoberbelin blue. Results showed that the proteinaceous nature of the Mn2+-oxidizing factor was not in accordance with the stimulation of Mn2+ oxidation by treatment with proteolytic enzymes.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Optimization of Cry3A yields in Bacillus thuringiensis by use of sporulation-dependent promoters in combination with the STAB-SD mRNA sequence
Article Abstract:
A study was conducted to show that Cry3A yields in Bacillus thuringiensis can be optimized using sporulation-dependent promoters during the inclusion of the STAB-SD sequence in the construct. The Expand Long Template PCR System was utilized to carry out polymerase chain reaction experiments. Four liquid media were then utilized to compare the bacterial strains. Moreover, sporulating cultures were observed with a Zeiss Photomicroscope III.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Production of an extracellular polyethylene-degrading enzyme(s) by Streptomyces species. Characterization of an extracellular protease inhibitor of Bacillus brevis HPD31 and nucleotide sequence of the corresponding gene
- Abstracts: Production of an extracellular polyethylene-degrading enzyme(s) by Streptomyces species. part 2 Changes in fatty acid branching and unsaturation of Streptomyces griseus and Brevibacterium fermentans as a response to growth temperature
- Abstracts: Induction of cellulase by gentiobiose and its sulfur-containing analog in Penicillium purpurogenum. Enigmatic gratuitous induction of the covalent flavoprotein vanillyl-alcohol oxidase in Penicillium simplicissimum
- Abstracts: Mesoderm induction and axis determination in Xenopus laevis. RNA-binding proteins: masking proteins revealed
- Abstracts: Stimulation of vesicular-arbuscular mycorrhizal fungi by mycotrophic and nonmycotrophic plant root systems. Inoculation of woody legumes with selected arbuscular mycorrhizal fungi and rhizobia to recover desertified Mediterranean ecosystems