Isolation and identification of methanogen-specific DNA from blanket bog peat by PCR amplification and sequence analysis

Article Abstract:

Methanogenic bacteria present in blanket bog peat are identified using PCR amplification and sequence analysis of the PCR products. The different 16S rRNA variants form two distinct groups within the methanogenic bacteria. The bacteria are present only in the lower soil sections as they are anaerobic in nature. DNA isolated from the soil is amplified with PCR probes specific for the archeal 16S rRNA gene or the methyl coenzyme M reductase gene. The PCR products are then separated and amplified further to analyze the gene insert sequences.

Analysis, DNA testing, DNA identification, Identification and classification, Polymerase chain reaction, Methanobacteriaceae, Methanogens, Peat-bogs, Peat bogs

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Effect of trophic status on the culturability and activity of bacteria from a range of lakes in the English Lake District

Article Abstract:

The influence of the existing lake conditions on the culturability and activity among different size classes of bacteria, was examined by using a series of lakes in the English Lake District. A steady transition from oligotrophic to eutrophic is displayed by the analyses based on planktonic variables.

Science & research, Influence, Bacteria, Lake ecology, Planktonic bacteria

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Direct measurements of natural planktonic bacterial community viability by flow cytometry

Article Abstract:

Fluorescent viability dyes in conjugation with flow cytometry (FCM) is a suitable technique for detection and viability assessment of specific components of freshwater bacteria. The cell permeability act as a limiting factor. The measurement of cell count using FCM and fluorescent dyes is discussed.

Flow cytometry, Marine bacteria

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