Isolation of a species-specific mitochondrial DNA sequence for identification of Tilletia indica, the Karnal bunt of wheat fungus

Article Abstract:

Primers from a cloned mitochondrial DNA fragment of Tilletia indica are useful in differentiating the Karnal bunt of wheat fungus, T. indica, from other Tilletia species using a polymerase chain reaction assay. The primer pair Ti1/Ti4 amplifies a 2.3 kb DNA fragment of T. indica isolates from India, Pakistan, and Mexico. The DNA isolates from other smut fungi such as T. barclayana, T. caries, and T. foetida are incapable of producing bands during the assay. The primers are useful in assays with DNA from teliospores infecting wheat seeds.

Diseases, Observations, Cloning, Wheat, Fungi, Pathogenic, Pathogenic fungi

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Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms

Article Abstract:

Characterization of attachedmicrobial communities was done in this study using the combined techniques of selective polymerase chain reaction (PCR) amplification, comparative sequencing and oligonucleotide probe hybridization. PCR amplification was selective but not specific. Visualization of specific sulfate-reducing bacterial population was provided by in situ hybridization with fluorescent oligonucleotide probes.

Physiological aspects, Bacteria, Microbial biotechnology, Bioreactors

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Identification of deoxynivalenol- and nivalenol-producing chemotypes of Gibberella zeae by using PCR

Article Abstract:

Researchers used PCR based on a primer pair derived from the Tri7 gene of Gibberella zeae to distinguish the two chemotypes based on trichothecene production. G. zeae H-11 produces deoxynivalenol and G. zeae 88-1 produces nivalenol. G. zeae causes cereal scab.

Fungi, Phytopathogenic, Phytopathogenic fungi, Trichothecenes

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