Kinase suppressor of Ras is ceramide-activated protein kinase

Article Abstract:

Immunoprecipitation and enzymatic analysis were performed to study and characterize the kinase suppressor of Ras (KSR) which has been previously identified in Caenorhabditis elegans and Drosophila as a ceramide-activated protein (CAP) kinase. The results confirmed that both KSR and CAP kinase are 100-kDa-active protein kinases that are membrane-bound and act upstream of Raf-1. It was also confirmed that the two proteins behave the same biochemical activities. The findings thus strongly suggest that KSR is a CAP kinase.

Usage, Phosphorylation, Cell culture, Tissue culture, Immunoassay, Enzymatic analysis

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Ceramide synthase mediates daunorubicin-induced apoptosis: an alternative mechanism for generating death signals

Article Abstract:

Ceramide generation mediates daunorubin-induced apoptosis. Ceramide synthase activity can be regulated in eukaryotes and constitute evidence that elevated ceramide is required for the induction of apoptosis. The anticancer drug daunorubicin stimulates ceramide elevation and apoptosis in P388 and U937 cells. Daunorubicin-stimulated ceramide elevation results from de novo synthesis via activation of the enzyme ceramide synthase.

Daunorubicin

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Acid sphingomyelinase-deficient human lymphoblasts and mice are defective in radiation-induced apoptosis

Article Abstract:

It has been hypothesized that sphingomyelinase activated by stress generates ceramide, the second messenger that initiates the apoptotic response. However, this hypothesis has never really been proven. To resolve this problem, a genetic approach is used to determine the validity of the hypothesis. Results conclusively show that acid sphingomyelinase is actively involved in a form of stress-induced apoptosis.

Research, Messenger RNA

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