Spontaneous formation of a mannitol-producing variant of Leuconostoc pseudomesenteroides grown in the presence of fructose

Article Abstract:

Researchers describe a strain of Leuconostoc pseudomesenteroides grown in the presence of fructose that produces mannitol. This is important because mannitol could be used in the food industry as a low-calorie sweetener, since it is not metabolized by humans.

Industrial microorganisms

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Metabolic engineering of mannitol production in Lactococcus lactis: Influence of overexpression of mannitol 1-phosphate dehydrogenase in different genetic backgrounds

Article Abstract:

The Lactococcus lactis produced by the mannitol is derived with the help of the mannitol1-phosphate dehydrgenase gene from lactobacillus planatarum which appears to a great extent in the wild type strain, a strain that lacks the lactate dehydrogenase (LDH) and a third type of strain that lessens the phosphofructokinase activity. Moreover metabolic engineering could be a useful element to attain mannitol production in the lactococcus lactis.

Analysis, Microbiology, Properties

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Overproduction of heterologous mannitol 1-phosphatase: A key factor for engineering mannitol production by Lactococcus lactis

Article Abstract:

The effect of overexpression of mannitol 1-phosphatase (M1Pase) gene from Eimeria tenella on the mannitol-producing capacities of Lactococcus lactis NZ9000, lactate dehydrogenase (LDH)-deficient L. lactis strain NZ9010, and strain HWA217 with reduced phosphofructokinase activity is investigated. The correlation between M1Pase activity and mannitol production is shown and the usefulness of this metabolic engineering approach is demonstrated.

Genetic aspects, Lactate dehydrogenase

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