Mitochondrial superoxide dismutase is essential for ethanol tolerance of Saccharomyces cerevisiae in the post-diauxic phase

Article Abstract:

The messenger RNA-superoxide dismutase (mRNA-SOD) levels and SOD activity in Saccharomyces cerevisiae cells were analyzed during cell growth to determine the role of SOD1- and SOD2-encoded proteins on stress tolerance. The activities of SOD1- and SOD2-encoded CuZnSOD and MnSOD increased in Saccharomyces cerevisiae during the exponential phase to the diauxic shift. The levels of mRNA-SOD1 and mRNA-SOD2 also increased during growth from the exponential to the diauxic shift phase. However, mRNA-SOD levels did not alter the sensitivity of the cells to ethanol.

Microbial growth, Microbial enzymes, Superoxide dismutase

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Near-UV-induced absorbance change and photochemical decomposition of ergosterol in the plasma membrane of the yeast Saccharomyces cerevisiae

Article Abstract:

The spectral profiles of cell suspensions and membrane fractions of Saccharomyces cerevisiae that were exposed to near-ultraviolet (UV) were analyzed to determine the components of the plasma membrane. Analysis of spectral absorbance changes in UV-exposed Saccharomyces cerevisiae cells indicated the presence of reduced levels of absorption spectra. The reduced levels of absorption spectra were also correlated to the chemical decomposition of ergosterol in the plasma membrane via a photochemical mechanism that was mediated by oxygen.

Ultraviolet radiation, Cell membranes, Plasma membranes, Sterols

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Comparative physiology of salt tolerance in Candida tropicalis and Saccharomyces cerevisiae

Article Abstract:

A comparison of the relative tolerance to osmotic stress and to alkali cation toxicity of the fermentive yeast Saccharomyces cerevisiae and the respiratory yeast Candida tropicalis was made. Glucose media distinguished that C. tropicalis has a poor adaptation to osmotic stress and exhibited reduced glycerol production compared to S. cerevisiae. Trehalose was also determined as an important osmoresponsive solute in S. cerevisiae in the absence of glucose repression.

Research, Sodium, Sodium (Chemical element), Glycerol, Osmosis

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