Effect of copper speciation on whole-cell soluble methane monooxygenase activity in Methylosinus trichosporium OB3b

Article Abstract:

A newly developed medium called M2M provides more bioavailable copper to soluble methane monooxygenase in Methylosinus trichosporium OB3b. This medium used pyrophosphate for metal chelation, whereas nitrate mineral salts use EDTA.

Oxidases, Culture media (Biology), Culture media, Copper, Copper (Nutrient), Bioavailability

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Mixed pollutant degradation by Methylosinus trichosporium OB3b expressing either soluble or particulate methane monooxygenase: Can the tortoise beat the hare?

Article Abstract:

The investigation of the biodegradation of mixtures of chlorinated solvents by Methylosinus trichosporium OB3b in the presence of methane using either form of monooxygenase (MMO) is presented. The results have indicated that the relative rates of growth substrate and pollutant degradation are key factors in selecting the correct form of MMO for pollutant degradation.

Science & research, Iowa, Chemical properties

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Methane and trichloroethylene degradation by Methylosinus trichosporium OB3b expressing particulate methane monooxygenase

Article Abstract:

A variation in the growth concentration of copper causes a change in the kinetics of methane and trichloroethylene (TCE) degradation. This constitutes evidence that copper has an important role in the ability of whole cells expressing particulate methane monooxygenase (pMMO) to degrade both methane and TCE. These whole-cell results indicate that the amount of available copper is crucial in determining the oxidation of substrates in methanotrophs expressing only pMMO.

Trichloroethylene

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