Quantitative detection of methanotrophs in soil by novel pmoA-targeted real-time PCR assays

Article Abstract:

Research has been conducted on methane oxidation in soils by methanotrophic bacteria. The authors report that the quantification of these bacteria in soil has become possible via the use of pmoA-targeted real-time PCR, and that this approach can be used for the quantitative analysis of the methanotroph community structure in nature.

Germany, Science & research, Analysis, Physiological aspects, Environmental aspects, Soils, Methane, Bacteria, Polymerase chain reaction, Microbiology, Oxidation-reduction reaction, Oxidation-reduction reactions, Chemical properties, Microbial ecology

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Molecular analyses of the methane-oxidizing microbial community in rice field soil by targeting the genes of the 16S rRNA, particulate methane monooxygenase, and methanol dehydrogenase

Article Abstract:

A denaturing gradient gel electrophoresis (DGGE) analysis of small-subunit (SSU) rRNA-based and functional gene markers was performed to examine changes in microbial community structure during induction of CH4 oxidation in rice field soil. The DGGE analysis employed a universal SSU rRNA-based primer set and two primer sets that target methylotrophic members of the gamma- and alpha-Proteobacteria, as well as primer sets that target functional genes. Results demonstrate that both type I and type II methanotrophs were present in the original rice field soil and populations of these methanotrophs apparently persisted even when CH4 oxidation activity already ceased.

Soil microbiology, Oxidation, Physiological, Physiological oxidation, Microbial populations

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