Identification of a Bacillus subtilis secretion mutant using a beta-galactosidase screening procedure

Article Abstract:

The mutant with conditional secretory defects in Bacillus subtilis has been identified by using the screening procedure for characterizing beta-galactorsidase (LacZ) fusion protein activity level. The growth of the mutant decreases after the production of LacZ at 42 degree celsius. At non-permissive temperature, the growth kinetics and secretion of Carlsberg-alkaline phosphate fusion product becomes defective.

Research, Analysis, Observations, Bacillus subtilis, Proteins, Mutation (Biology), Mutation

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The extracytoplasmic folding factor PrsA is required for protein secretion only in the presence of the cell wall in Bacillus subtilis

Article Abstract:

Results reveal that the extracytoplasmic folding factor PrsA is essential for for the folding , stability or secretion of the exported proteins and functions as a chaperone protein. Data indicate that cell wall plays an important role in protein secretion as seen in the release of AmyQ alpha-amylase from the cell membrane in a model system.

Finland, Physiological aspects, Secretion, Biological transport, Cellular control mechanisms, Cell regulation, Protein folding, Plant cell walls, Cell walls

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