Molecular characterization of an Aspergillus parasiticus dehydrogenase gene, norA, located on the aflatoxin biosynthesis gene cluster

Article Abstract:

The coding region of the dehydrogenase gene norA from the fungus Aspergillus parasiticus contains 388 residues that encode a protein of 43 kDa. The norA gene is 49% identical to the aryl-alcohol dehydrogenase gene of Phanerochaete chrysosporium. The encoded protein has a norsolorinic acid reductase (NOR) activity. The norA gene is involved in aflatoxin biosynthesis, which is necessary for the transcription of norA. The cDNA clone was isolated with monoclonal antibodies acting against the purified A. parasiticus protein, with NOR activity.

Analysis, Microbial enzymes, Amino acid sequence, Amino acid sequencing

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Culture conditions control expression of the genes for aflatoxin and sterigmatocystin biosynthesis in Aspergillus parasiticus and A. nidulans

Article Abstract:

The effects of culture temperature and nitrate on gene expression for sterigmatocystin biosynthesis in Aspergillus parasiticus and Aspergillus nidulans were investigated. Experiments were conducted to determine if mycotoxins sterigmatocystin and aflatoxin transcripts in Aspergillus parasiticus and Aspergillus nidulans are differentially transcribed. Results showed that high temperature and nitrate induced gene expression for sterigmatocystin biosynthesis. It was also found that the two mycotoxins are regulated differently.

Physiological aspects, Genetic regulation, Mycotoxins, Nitrates, High temperatures

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Molecular cloning of genes related to aflatoxin biosynthesis by differentialscreening

Article Abstract:

A molecular cloning procedure called differential hybridization was used in cloning aflatoxin genes from Aspergillusparasiticus. This procedure made use of three influencing factors namely growthphase, carbon source and temperature which induced differential gene expression Nineteen clones enriched for genes related to aflatoxin biosynthesis were generated. Characterization of the clones by Northern hybridization showed further correlation of the clones with aflatoxin biosynthesis.

Methods, Cloning, Microbial genetics

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