Molecular cloning and mapping of phenol degradation genes from Bacillus stearothermophilus FDTP-3 and their expression in Escherichia coli

Article Abstract:

Phenol degradation in Bacillus stearothermophilus FDTP-3 takes place as a two-stage process via the meta pathway. Two genes coding for both enzymes of the meta pathway, namely phenol hydroxylase and catechol 2,3-dioxygenase, were cloned and mapped. The enzyme encoded by a plasmid tagged pFDA17 was found to be more thermostable than that encoded for by pTG402. The pFDA17 dioxygenase gene was found to share limited homology with the xylE gene of Pseudomonas putida.

Microbial metabolism, Chromosome mapping

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Mathematical models for the effects of pH, temperature, and sodium chloride on the growth of Bacillus stearothermophilus in salty carrots

Article Abstract:

Germination, outgrowth and lag (GOL) time and the exponential growth rate (EGR) of Bacillus stearothermophilus in salty carrot medium were characterized and modeled as a function of pH, temperature and NaCl concentration. Quadratic polynomial models for GOL time and EGR of B. stearothermophilus in terms of temperature, pH and NaCl were produced by response surface analysis. The r2 values for the GOL time and EGR models were 0.917 and 0.916, respectively.

Models, Bacterial growth

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Effect of calcium in assay medium on D value of Bacillus stearothermophilus ATCC 7953 spores

Article Abstract:

The calcium concentration of assay media can increase the D value of Bacillus stearothermophilus ATCC 7953 spores. The D value is a measure of the resistance of bacterial spores to sterilization. Calcium can affect the heat resistance of bacterial spores.

Physiological aspects, Culture media (Biology), Composition, Culture media, Calcium, Calcium (Chemical element), Sterilization (Disinfection), Sterilization

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