Monoclonal antibodies that react with live Listeria spp
Article Abstract:
Seven monoclonal antibodies (MAbs) against Listeria spp. reactive with live Listeria spp. were developed, two of which (55.8 and 55-37) were members of the immunoglobin M class, while the others were members of the immunoglobin G class. MAb 55-23 reacted with 148 of 157 strains tested while MAb 34-51 reacted with serotype 1/2a, 1/2b and 1/2c strains demonstrating a scattered reaction pattern with strains of other serotype 4b tested. The reactivities of MAbs were determined by a magnetic immunoluminescence assay.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Rapid detection of salmonellae in poultry with the magnetic immuno-polymerase chain reaction assay
Article Abstract:
The rapid detection of salmonellae in poultry by using the magnetic immuno-polymerase chain reaction assay (MIPA) was reported. The salmonellae present on 25 g of spiked chicken meat were enriched for 24 h in either buffered peptone water or selenite-cystine broth before detection by the MIPA. It was shown that when the MIPA is used in combination with enrichment, approximately 0.1 colony forming unit of Salmonella spp. per g of meat can be detected within 30 h.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Monoclonal antibodies that detect live salmonellae
Article Abstract:
The production of serotype-specific monoclonal antibodies that bind to live or intact salmonellae belonging to serogroups A, B, C1, C2, D, E and K and Salmonella choleraesuis subsp. diarizonae are discussed. Magnetic immunoluminesence assay and dot spot analysis were used to detect clinical isolates belonging to this Selmonella group. It was found that each monoclonal body was reactive with a distinct serotype.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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