Mutational analysis of pcpA and its role in pentachlorophenol degradation by Sphingomonas (Flavobacterium) chlorophenolica ATCC 39723

Article Abstract:

Research was conducted to study the disruption of the genomic pcpA gene by gene targeting through the characterization of the mutated gene, homologous recombination, and isolation of pentachlorophenol (PCP) metabolites produced by the mutant strains of Sphingomonas chlorophenolica ATCC 39723 containing the disrupted gene. Cells were washed with sterile deionized water and sterile glycerol. Total genomic DNA was isolated based on the DNAZOL guanidine-detergent lysis method. Moreover, hydroquinone was separated from neutral extracts of cultures incubated with PCP.

Gene mutations, Gene mutation, Chlorophenols

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Accelerated mineralization of pentachlorophenol in soil upon inoculation with Mycobacterium chlorophenolicum PCP1 and Sphingomonas chlorophenolica RA2

Article Abstract:

The inoculation of nonsterile soil from a pentachlorophenol (PCP) contaminated site with Sphingomonas chlorophenolica RA2 accelerates mineralization of PCP as compared to inoculation with Mycobacterium chlorophenolicum. The mineralization of PCP by both bacteria is more at lower concentrations of PCP in soil. At higher concentrations of PCP, the activity of both strains is inhibited. Structural changes with sawdust and cell attachment to polyurethane alters rates of mineralization significantly in the presence of high concentrations of PCP.

Physiological aspects, Mycobacterium, Biomineralization

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Reductive dechlorination of chlorophenols by a pentachlorophenol-acclimated methanogenic consortium

Article Abstract:

The observed pathways for the reductive dechlorination of pentachlorophenol were described. An unacclimated methanogenic consortium removed chlorines from the position ortho to the hydroxyl group while the acclimated anaerobic consortium removed chlorines from thhe ortho, meta and para positions. Thus, the acclimated consortium yielded biotransformation pathways different from those of unacclimated consortium.

Biodegradation, Methanobacteriaceae, Methanogens

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