Mutations in the second-largest subunit of Drosophila RNA polymerase II interact with Ubx

Article Abstract:

Particular mutations in RpII215, the gene coding for the largest subunit of the Drosophila RNA polymerase II also causes the third thoracic segment to take the form of the second segment. Mutation in the third thoracic segment can also be induced by decreasing the concentration of Ultrabithorax (Ubx). The recessive lethal mutations in the region coding for the second largest subunit of the RNA polymerase II can be assigned to 13 complementation groups, three of which, when combined with low levels of Ubx can cause abnormalities in the third thoracic segment similar to mutations produced by alterations in RpII215.

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Reverse genetics of Drosophila RNA polymerase II: identification and characterization of RpII140, the genomic locus for the second-largest subunit

Article Abstract:

The reverse genetics approach was used to isolate genes for two subunits of Drosophila melanogaster RNA polymerase II. The origins of RpII180 and RpII40 were genomic rather than operon based. This differentiated them from similar genes of some bacterial polymerases. When coding regions were deleted from RpII140, the transformed structure did not rescue A5 alleles but was able to rescue Z6 alleles. This showed a direct relationship between A5 complementary alleles and the genomic RpII140 locus.

Analysis, Usage, Genetic transcription, Transcription (Genetics), Chromosome mapping

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Functional studies of the carboxy-terminal repeat domain of Drosophila RNA polymerase II in vivo

Article Abstract:

RNA polymerase II activities are examined in Drosophila RpII215 mutants at various carboxy-terminal repeat domain (CTD) levels. Wild-type levels are approximated by steady-state mRNA levels from transgenes encoded with a completely truncated CTD-less subunit. Elimination of the total CTD generated polymerase completely defective in vivo, while partial CTD removal generated substantial in vivo activity.

Mutation (Biology), Mutation

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