Nbs1 potentiates ATP-driven DNA unwinding and endonuclease cleavage by the MRe11/Rad50 complex

Article Abstract:

The Nijmegen breakage syndrome gene product (Nbs1) potentiates ATP-driven endonuclease cleavage and DNA unwinding by the MRe11/Rad50 complex. The triple complex of recombinant Mre11, Nbs1and Rad 50 proteins binds cooperatively to DNA. A distinct protein-DNA species is formed. The complex has several enzymatic functions not seen in the absence of Nbs1. On nonhairpin DNA ends, ATP regulates a switch in endonuclease specificity that allows Mre11/Rad50/Nbs1 to cleave a 3'-protruding strand at a transition from a double to a single strand. Mutational analysis shows Rad50 to be responsible for ATP binding by the complex. The ATP-dependent activities are seen only with Nbs1.

Analysis, Genetic recombination, Adenosine triphosphate, ATP, DNA binding proteins, Genetic research

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The control of Spo11's interaction with meiotic recombination hotspots

Article Abstract:

The activity of the evolutionary conserved topoisomerase homolog Sp011 initiates the meiotic recombination by programmed double -strand break (DSBs). Detection is done on the transient, noncovalent association of Spo11 with meiotic hotspots in wild-type cells by using chromatin-immunoprecipitation (ChIP).

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A 5' RNA element promotes dengue virus RNA synthesis on a circular genome

Article Abstract:

A demonstration that RNA-RNA interactions between 5' and 3' end sequences of the viral genome enhance dengue virus RNA synthesis only in the presence of an intact SLA is presented. An explanation for the strict requirement of dengue virus genome cyclization during viral replication is presented.

Genetic aspects, RNA, Cell interaction, Cell interactions, Dengue viruses, Dengue virus, RNA synthesis

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