Nuclear mutations in the petite-negative yeast Schizosaccharomyces pombe allow growth of cells lacking mitochondrial DNA

Article Abstract:

Strains of Saccharomyces pombe lacking mitochondrial DNA (mtDNA) were developed by very long term treatment with glucose, potassium acetate and ethidium bromide. However, induction of this rho(super o) genotype was not reproducible from culture to culture. Further genetic analysis showed that rho(super o) depends on nuclear mutations in at least two unlinked genes, designated ptp1-1 and ptp2-1, standing for petite-positive. No other phenotypic traits were associated with the ptp mutations in mtDNA-containing strains.

Microbiology, Cultures (Biology)

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PET111 acts in the 5'-leader of the Saccharomyces cerevisiae mitochondrial COX2 mRNA to promote its translation

Article Abstract:

The yeast nuclear gene PET111 is essential for the expression of the mitochondrial gene COX2 which encodes cytochrome c oxidase subunit II or coxII. The site of PET111 action was studied in vitro by introducing genes coding for chimeric mRNAs into mitochondria through transformation and by observing their expression. Results showed that PET111 acts in the short COX2 5'-leader mRNA to activate the translation of the downstream coding sequences. Moreover, PET111 was not required in any post-translational step.

Messenger RNA, Genetic translation, Translation (Genetics)

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Mutations in the mitochondrial ATP synthase gamma subunit suppress a slow-growth phenotype of yme1 yeast lacking mitochondrial DNA

Article Abstract:

Second site suppressor strains bearing slow-growth phenotype were isolated from yme1 rhodegree yeast. The strains exhibited a dominant suppressing mutation in the gamma subunit of the mitochondrial ATP synthase. Mitochondrial DNA has functional value for yme1 mutant despite the absence of electron transport and oxidative phosphorylation. Suppressor analysis showed that the sampled mutations hindered the rhodegree slow-growth phenotype.

Phenotype, Phenotypes, Mutation (Biology), Mutation, Adenosine triphosphatase

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