On-line detection of substrate exhaustion by using NAD(P)H fluorescence

Article Abstract:

Aerobic fermentations of the threonine auxotroph Corynebacterium glutamicum were monitored by NAD(P)H fluorescence. Resulting information from the on-line fluorescence signal, such as the rate of change of the fluorescence signal, wasused to evaluate fermentation-related culture metabolic activities, instead of mathematical corrections for inner filter effects. The accuracy of the filteredfluorescence change rate signal for predicting growth-essential substrate depletion was demonstrated.

Methods, Fermentation, Fluorescence

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Cloning of the trp gene cluster from a tryptophan-hyperproducng strain of Corynebacterium glutamicum: identification of a mutation in the trp leader squence

Article Abstract:

Tryptophan biosynthesis gene clusters of the Corynebacterium glutamicum strain were cloned and characterized from Escherichia coli. Resistance of E. coli strains to tryptophan logs was demonstrated. A comparison is made between the amino acid sequence deduced by trpe gene with those encoded from other bacteria. Results indicate the occcurrence of a nonsense mutation which causes constitutive antitermination response.

Genetic aspects, Microbial mutation, Tryptophan

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Production of (R)-3-chloro-1,2-propanediol from prochiral 1,3-dichloro-2-propanol by Corynebacterium sp. strain N-1074

Article Abstract:

The utilization of the bacterial strain Corynebacterium in examining the production of (R)-3-chloro-1,2-propaniedol (MCP) from prochiral 1,3-dichloro-2-propanol (DCP) is discussed. DCP was transformed into MCP by theaddition of glycerol as a carbon source or some chlorinated alcohols to a medium. Results indicate that the formation of enzyme activity can be induced by some metabolites of chlorinated alcohols.

Glycerol

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