Photometric application of the Gram stain method to characterize natural bacterial populations in aquatic environments

Article Abstract:

Photometric Gram stain images were characterized by using the Gram stain index (GSI) to specify aquatic bacterial populations. Escherichia coli was used as the standard of the typical gram-negative bacteria, while Bacillus subtilis was used as the standard of the typical gram-positive bacteria. The findings show a distinct difference between the GSI spectra of E. coli and that of B. subtilis. The range of the GSI of B subtilis cells was broader and its maximal peak was lower.

Usage, Bacillus subtilis, Aquatic microbiology, Microbial populations, Photometry

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Biotinylation facilitates the uptake of large peptides by Escherichia coli and other gram-negative bacteria

Article Abstract:

The study demonstrated that biotinylation can facilitate the uptake of peptides up to 31 amino acids in length by Escherichia coli and that transport is dependent on the biotin transporter, BirB or BioP. It is concluded that uptake of biotinylated peptides could be competitively inhibited by free biotin or avidin and blocked by the protonophore CCCP, which disrupts membrane potential.

Peptides

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Lactobacillus casei DN-114 001 inhibits the ability of adherent-invasive escherichia coli isolated from Crohn's disease patients to adhere to and to invade intestinal epithelial cells

Article Abstract:

Ileal lesions in 36,4% of patients with Crohn's disease are colonized by pathogenic adherent-invasive Escherichia coli. This study determined the in vitro inhibitory effects of the probiotic strain, Lactobacillus casei DN-114 001, on adhesion to and invasion of human intestinal epithelial cells by adherent-invasive Escherichia coli isolated from Crohn's disease patients.

Epithelial cells, Crohn's disease

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