Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNA and amoA sequence analysis: implications for molecular diversity surveys

Article Abstract:

Comparative 16S rRNA and amoA sequence analysis was used to study the molecular diversity of ammonia-oxidizing bacteria. Using this technique, 122 amoA sequences from 11 nitrifying wastewater treatment plants showed the only nitrosomonads existed in most plants.

Biodegradation

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Community structure and activity dynamics of nitrifying bacteria in a phosphate-removing biofilm

Article Abstract:

Analysis of bacteria in a phosphate-removing biofilm from a sequencing batch biofilm reactor shows three species of Nitrosomonas as the predominant organisms. N. europaea and N. oligotropha predominate in the first 100 micrometers, with N. oligotropha found almost exclusively in deeper layers of the biofilm.

Microbial mats, Water, Water treatment

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Combination of fluorescent in situ hybridization and microautoradiography - a new tool for structure-function analyses in microbial ecology

Article Abstract:

The performance of fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes and microautoradiography for the identification and determination of substrate uptake patterns of individual microbial cells has been assessed. The method was tested using defined artificial mixtures of Escherichia coli and Herpetosiphon aurantiacus under aerobic conditions in the presence of (3H) glucose. Results indicate that the method can be used in identifying the uptake of labeled substrates for defined cell mixtures and activated sludge.

Analysis, Usage, Evaluation, In situ hybridization, Microbial populations, Microbiological assay

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