Polynucleotide probes that target a hypervariable region of 16S rRNA genes to identify bacterial isolates corresponding to bands of community fingerprints

Article Abstract:

The feasibility of using the hypervariable region V6 of the small-subunit rRNA gene as a probe target in detecting bacterial isolates corresponding to bands of temperature gradient gel electrophoresis (TGGE) community fingerprints has been studied. Polymerase chain reactions were used in deriving digoxigenin-labeled probes, using bands from TGGE fingerprints as templates. The latter are used in hybridizations with dot blotted 16S rDNA from bacterial isolates. Results indicate that the V6 region meets the criteria for high phylogenetic variability which is needed for probe specificity.

DNA testing, DNA identification, Nucleic acid probes

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Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients

Article Abstract:

Two methods of studying actinomycete populations in their natural environments. Actinomycetes are gram-positive bacteria that are involved in the degradation of organic matter in soil including polymers and urban wastes. A forward primer to selectively amplify 16S rDNA of actinomycetes by PCR was constructed and used with various reverse primers. Temperature or denaturing gradient gel electrophoresis was used to study fragments of actinomycete-specific polymerase chain reaction.

Physiological aspects, Bacteria, Microbial populations

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Repeat domain diversity of avrBs3-like genes in Ralstonia solanacearum strains and association with host preferences in the field

Article Abstract:

Genes homologues to avrBs3 of Xanthomonas within the R. solanacearum species complex and the size of the repeat region correlated with host preferences of the strains are investigated. It is observed that the sequences of repeats and variation between nearly clonal strains reveal evidence of frequent recombination.

Biological diversity, Biodiversity, Homology (Biology), Xanthoma

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