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Production of minicellulosomes from Clostridium cellulovorans in Bacillus subtilis WB800

Article Abstract:

The two gene encoding EngB endoglucanase and mini-CbpA1 scaffolding protein of Clostridium cellulovorans are constructed and coexpressed in Bacillus subtilis. The in vivo synthesis of minicellulosomes suggests that it is possible to insert into B. subtilis cellulosomal genes that would allow growth on cellulosic materials and the production of various designer cellulosomes with specific functions.

Author: Inui, Masayuki, Yukawa, Hideaki, Hee-Yeon Cho, Sui-Lam Wong
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
Genetic aspects, Bacillus subtilis, Clostridium botulinum, Microbiological research

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Manipulating corynebacteria, from individual genes to chromosomes

Article Abstract:

The various recombinant DNA tools that are available for genetic manipulations of corynebactria, with an emphasis on Corynebacterium glutamicium are presented. The efficiency of microbial technology should be optimized in order to produce commodity biochemicals and expand the industrial scope and societal impact of biotechnology.

Author: Inui, Masayuki, Yukawa, Hideaki, Vertes, Alain A.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
Science & research, Biological Product (except Diagnostic) Manufacturing, Drugs, Recombinant DNA Products, Research, Recombinant DNA, Chromosomes, Corynebacteria

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