Protein splicing of inteins and hedgehog autoproteolysis: structure, function, and evolution

Article Abstract:

A research was conducted to study the protein splicing of inteins and hedgehog autoproteolysis using several approaches that defined the splicing mechanism and the three-dimensional structure of the Saccharomyces cerevisiae VMA intein. The structure of the Drosophila hedgehog protein autoprocessing domain was compared with the splicing domain. Results showed that large inteins were characterized by homing endonucleases that represent a core endonuclease domain.

Genetic aspects, Saccharomyces, Proteolysis, Protein separation

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Bacteriophage-based genetic system for selection of nonsplicing inteins

Article Abstract:

A genetic selection system that detects splicing and nonsplicing activities of inteins is constructed based on the ability to rescue a T4 phage strain with a conditionally inactive DNA polymerase. The conclusion suggest that development of genetic systems with the potential of identifying protein splicing inhibitors is a first step towards controlling proliferation of pathogenic microbes harboring inteins in essential proteins.

Risk factors, DNA polymerases, Bacteriophage T4

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Experimental and theoretical bases of specific affinity, a cytoarchitecture-based formulation of nutrient collection proposed to supercede the Michaelis-Menten paradigm of microbial kinetics

Article Abstract:

A relationship between the concentration-limited rate of nutrient transport and organism cytoarchitecture based on available theory, in situ and laboratory kinetic data and derived concepts are described. The results obtained are in contrast to the Michaelis-Menten theory and they demonstrated that most oligobacteria in the environment use multiple substrates simultaneously to attain sufficient energy and material for growth.

Microbiological research

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