Ultrastructural and immunocytochemical studies on the H2O2-producing enzyme pyranose oxidase in Phanerochaete chrysosporium grown under liquid culture conditions

Article Abstract:

Enzymatic, immunocytochemical andultrastructural studies were conducted to determine the intra- and extracelllular distribution of pyranose 2-oxidase (POD) in cultures of Phanerochaete chrysosporium K-3. The results showed that POD was preferentiallydistributed to intracellular membrane structures in electron-lucent vesicles, multivesicular bodies and in the periplasmic space of hyphae. The enzyme was also detected extracellularly. These results indicate that POD may be an important donor of hydrogen peroxide for hydrogen peroxide-dependent peroxidases during lignocellulose degradation.

Physiological aspects, Hydrogen peroxide, Peroxidase

---

Purification by immunoaffinity chromatography, characterization, and structural analysis of a thermostable pyranose oxidase from the white rot fungus Phlebiopsis gigantea

Article Abstract:

The pyranose oxidase (PROD) with a relative molecular weight of nearly 295,600 has been purified by heating, immunoaffinity chromatography and gel filtration. PROD, produced by the white rot fungus Phlebiopsis gigantea, is thermostable, yellow in color, and has a pI between pH 5.3 and 5.7. PROD is a tetrameric flavoprotein composed of ellipsoid subunits. Flavin adenine dinucleotides are covalently bound to PROD. The enzyme acts on D-glucose, L-sorbose and D-xylose, and is stable during storage.

Analysis, Molecular structure

---

Similar abstracts:

• Abstracts: Purification and characterization of cellobiose dehydrogenase from the plant pathogen Sclerotium (Athelia) rolfsii
• Abstracts: Cloning and characterization of three fatty alcohol oxidase genes from Candida tropicalis strain ATCC 20336. Transformation of fatty acids catalyzed by cytochrome P450 monooxygenase enzymes of Candida tropicalis
• Abstracts: Cloning and characterization of a gene (LIP1) which encodes a lipase from the pathogenic yeast Candida albicans
• Abstracts: Genetic diversity of Macaronesian leafy liverwort Porella canariensis inferred from RAPD markers. Non-sex-linked, nuclear cleaved amplified polymorphic sequences in Silene latifolia
• Abstracts: Spatial distribution of marine crenarchaeto group I in the vicinity of deep-sea hydrothermal systems. Distribution of archaea in a black smoker chimney structure

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.