Quantitative determination of the spatial distribution of Nitrosomonas europaea and Nitrobacter agilis cells immobilized in Kappa-carrageenan gel beads by a specific fluorescent-antibody labelling technique

Article Abstract:

The spatial distribution of two microorganisms in a biofilm is determined by using a novel technique which combines labelling and stereological methods. Cells of Nitrosomonas europaea (ATCC 19718) and Nitrobacter agilis (ATCC 1413) are homogeneously distributed in a kappa-carrageenan gel during immobilization, and they grew out to colonies. A biomass volume distribution for N. europea and N. agilis in kappa-carrageenan gel beads is determined from the positions and surface of the colonies of each species.

Distribution, Cells (Biology), Cells

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Investigation of an acetate-fed denitrifying microbial community by stable isotope probing, full-cycle rRNA analysis, and fluorescent in situ hybridization-microautoradiography

Article Abstract:

The acetate-utilizing microbial consortium in a full-scale activates sludge process is determined without prior enrichment using stable isotope probing (SIP), full-cycle rRNA analysis and fluorescent in situ hybridization (FISH)-microautoradiography. A combination of molecular methods have indicated that bacteria related to the genera Acidovorax, Dechloromonas and Thauera belonging to the families Comamonadaceae and Rhodocyclaceae are capable of utilizing acetate under anoxic conditions.

United States, Science & research, In situ hybridization

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Cloning, nucleotide sequence, and regulatory analysis of the Nitrosomonas europaea dnaK gene

Article Abstract:

Cloning and sequencing of the danK gene of Nitrosomonas europaea indicated the homology of the heat shock protein product's amino acid sequence to the dnaK gene of other organisms. Nucleotide sequencing of the dnaK gene also indicated the presence of a functional heat shock promoter that is similar to the sigma promoters of other gram-negative bacteria. Furthermore, the dnaK gene exhibited two transcription start sites in the heat shock-induced cell.

Genetic aspects, Gram-negative bacteria, Heat shock proteins, Promoters (Genetics), Microbial genetics

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