Quantitative immunocapture PCR assay for detection of Campylobacter jejuni in foods

Article Abstract:

Research describes the development of an immunocapture PCR technique for the detection of Campylobacter jejuni in contaminated foods. Results show that the technique enables direct detection of the bacteria without enrichment and takes approximately 8h. The assay detects one cell in one milliliter of sample.

Food, Food microbiology, Bacteria, Pathogenic, Pathogenic bacteria, Bacteriology

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Determination of the sensitivity of a rapid Escherichia coli O157:H7 assay for testing 375-gram composite samples

Article Abstract:

Research describes the development of an immunoassay for the detection of Escherichia coli in ground beef samples. Data indicate that a combination of an immunomagnetic separation technique and an enzyme-linked immunosorbent assay detects very low levels of E.coli O157:H7 in ground beef samples.

Research, Escherichia coli, Beef, Contamination, Immunochemistry

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Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment, permitting detection of Pneumocystis carinii DNA

Article Abstract:

A technique is presented for DNA capture in outdoor air samples which removes environmental inhibitors, thereby preserving the sensitivity of the 16S polymerase chain reaction method for Pneumocystis carinii. Its application in health care for those susceptible to pneumonia is also discussed.

Methods, Prevention, Air pollution control, DNA testing, DNA identification, Pneumonia, Air quality management, Air quality, Pneumocystis carinii

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