RNA-dependent replication, transcription, and persistence of Brome mosaic virus RNA replicons in S. cerevisiae

Article Abstract:

The RNA-dependent replication, transcription and persistence of Brome mosaic virus (BMV) RNA derivatives carrying foreign genes in Saccharomyces cerevisiae transformed with the BMV RNA replication genes 1a and 2a are reported. This proves that it is possible to use S. cerevisiae containing the necessary replication and transcription factors for the replication and transcription of viruses of higher eukaryotic organisms. S. cerevisiae thus represents a powerful medium for studying virus-host interactions in higher eukaryotes.

Research, Methods, Usage, Viral research, Saccharomyces, Virus research, Host-virus relationships

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Frpo: a novel single-stranded DNA promoter for transcription and for primer RNA synthesis of DNA replication

Article Abstract:

A new single-stranded DNA promoter functions effectively for E.coli RNA polymerase. Through the stimulation of SSB, the new single stranded DNA promoter (Frpo sequence) instructs the RNA polymerase to begin transcription at a definite site within Frpo. Transcription at Frpo also functions for preparing DNA replication since RNAs synthesized on SSB-coated single-stranded Frpo are practically elongated into DNA chains by DNA polymerase III holoenzyme.

DNA, RNA, RNA synthesis

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Structure and replication of yeast prions

Article Abstract:

A model that illustrates the role played by Hsp104p in propagating the prion structure of the yeast Sup35 protein is developed. Yeast determinants [PSI+] and [URE3] have been observed to exhibit unusual genetic attributes such as non-Mendelian, cytoplasmic inheritance. In this model, Hsp104p facilitates the folding of Sup35 in a prion-like structure. In addition, it cleaves Sup35p aggregates into smaller pieces.

Models, Analysis, Proteins, Prions, Prions (Proteins)

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