Rapid invasion by colicinogenic Escherichia coli with novel immunity functions

Article Abstract:

An experimental method was used to evaluate the likelihood of the proposed 'diversifying selection' hypothesis of colicin gene cluster evolution in Escherichia coli. Invasion experiments were conducted by introducing dual- and single-immunity into established colicinogenic populations. Results revealed that invasion by strains expressing novel immunity functions occurred rapidly, primarily due to the colicin killing effect. Further, the dual-immunity strain can invade a single immunity colicinogenic population rapidly in LB culture.

Genetic aspects, Escherichia coli, Biological invasions, Cellular immunity

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Structure and expression of the fliA operon of Salmonella typhimurium

Article Abstract:

A study was conducted to analyze the DNA sequence and transcriptional control of the fliA operon of Salmonella typhimurium. A disruption mutant was constructed by inserting a kanamycin-resistant gene cassette into the fliZ gene on the chromosome. Invitro RNA synthesis was then performed using Escherichia coli RNA polymerase. Results indicated that the fliZ gene is needed for the expression of maximal motility.

DNA, Operons, RNA polymerases, Salmonella typhimurium

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