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Real-time fluorogenic reverse transcription-PCR assays for detection of bacteriophage MS2

Article Abstract:

A study is conducted on the detection of bacteriophage MS2 using real-time flourogenic reverse transcription-PCR assays, for studying environmental transport and fate of pathogenic viruses in groundwater. Assays for nonpathogenic microorganisms such as those described in the study provide a safer, cost-effective means of testing such equipment in advance of validation with live pathogens.

Author: O'Connell, Kevin P., Bucher, Jennifer R., Anderson, Patricia E., Cheng J. Cao, Khan, Akbar S., Gostomski, Mark V., Valdes, James J.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
Bacteriophages, Genetic transcription, Transcription (Genetics)

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Phylogenetic characterization of 16S rRNA gene clones from deep-groundwater microorganisms that pass through 0.2-micrometer-pore-size filters

Article Abstract:

A total of 247 clones of 16S rRNA genes from microorganisms captured by 0.2-and 0.1-Mu m-pore-size filters from sedimentary and granite rock aquifers were amplified and yielded 37 operational taxonomic units (OTUs). OTUs capture by 0.2-Mu m-pore-size filters were largely affiliated with Betaproteobacteria.

Author: Naganuma, Takeshi, Miyoshi, Tatsuo, Iwatsuki, Teruki
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
Ribosomal RNA, Cladistic analysis

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Transcriptional organization of genes for protocatechuate and quinate degradation from Acinetobacter sp. strain ADP1

Article Abstract:

The data suggesting the existence of a large 14-kb primary transcript covering the pca and qui genes is reported. The respective genes are part of the chromosomal dca-pca-qui-pob-hca cluster encoding these pathways.

Author: Dal, Sureyya, Trautwein, Gaby, Gersicher, Ulrike
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
Adenosine diphosphate

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Subjects list: Research, Genetic aspects, Microorganisms
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