Sensitive quantitative detection of commensal bacteria by rRNA-targeted reverse transcription-PCRSensitive quantitative detection of commensal bacteria by rRNA-targeted reverse transcription-PCR
Article Abstract:
The viable counts for many commensal bacteria species including Escherichia coli were generated using an rRNA-targeted reverse transcription-polymerase chain reaction (RT-qPCR). The results have shown that RNA-targeted RT-qPCR assays have provided a convenient system for quantification of commensal bacterial and also for examining their possible invasion of a host.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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In vivo functional assay of a recombinant aquaporin in Pichia pastoris
Article Abstract:
The PvTIP3;1 is a plant aquaporin protein, which is strictly selective for water. To investigate its water channel properties, an overexpression system in Pichia pastoris was developed and devised in, in vivo assay of aquaporin function and the Escherichia coli glycerol channel GlpF that served as a negative control for aquaporin function was also expressed.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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The presence of N-terminal secretion signal sequences leads to strong stimulation of the total expression levels of three tested medically important proteins during high-cell-density cultivations of Escherichia coli
Article Abstract:
The use of the pJB658-based expression system to produce granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon-[alpha]2b (IFN-[alpha]2b), and single-chain antibody variable fragment (scFv-phOx) both intracellularly lacking signal sequences and through secretion with signal sequences during high-cell-density cultivation is described.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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