Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR
Article Abstract:
A fast and simple method for the quantitative detection of Listeria monocytogenes in meat products based on filtration, Chelex-100-based DNA purification, and real-time PCR is described. It is demonstrated that the method can detect as few as 100 CFU/g and quantify as few as 1000 CFU/g, with excellent accuracy compared to that of the plate count method and thus it is a promising alternative for the detection of L. monocytogenes in meat products.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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Internally controlled real-time PCR method for quantitative species-specific detection and vapA genotyping of Rhodococcus equi
Article Abstract:
A novel quantitative real-time PCR (Q-PCR) method was developed for the soil actinomycete Rhodococcus equi, an important horse pathogen and emerging human pathogen. It if found that the dual-reaction Q-PCR system offers a useful tool to both medical and veterinary diagnostic laboratories for the quantitative detection of R. equi and (optional) [vapA.sup.+] 'horse pathogenic' genotype determination.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Quantitative detection of Listeria monocytogenes and Listeria innocua by real-time PCR: assessment of hly, iap, and lin02483 targets and Amplifluor technology
Article Abstract:
The usefulness of hly and iap genes as targets for the specific quantitative detection of L. monocytogenes by RTi-PCR is evaluated. The Amplifluor and TaqMan technologies are used in the experiment.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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