Production of poly(3-hydroxybutyrate) by fed-batch culture of recombinant Escherichia coli with a highly concentrated whey solution

Article Abstract:

Researchers describe a recombinant form of E. coli that can produce poly(3-hydroxybutyrate) from whey. PHAs are the subject of intense research because they are a biodegradable plastic that can be produced by many bacteria. Growing the bacteria on whey would provide a use for whey, half of which is discarded as waste.

Whey, Polyhydroxybutyrate

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Recovery and characterization of poly (3-hydroxybutyric acid) synthesized in Alcaligenes eutrophus and recombinant Escherichia coli

Article Abstract:

Use of a sodium hypochlorite solution or dispersions of a sodium hypochlorite solution and chloroform helps recover poly(3-hydroxybutyric acid) (PHB) from Alcaligenes eutrophus and a recombinant Escherichia coli strains. Use of sodium hypochlorite for recovery leads to higher degradation of PHB in A. eutrophus compared to the amount degraded in recombinant E. coli strains. Examination of the purified PHB reveals the crystalline nature of PHB in the recombinant E. coli strains, while PHB exists in an amorphous state in A. eutrophus. This crystalline morphology accounts for the higher stability of native PHB in the recombinant E. coli strains.

Analysis, Biodegradation, Marine bacteria

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High-level production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by fed-batch culture of recombinant Escherichia coli

Article Abstract:

High-level production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) is discussed as carried out through fed-batch culture of recombinant Escherichia coli. High production cost is a difficulty in commercializing polyhydroxyalkanoates (PHAs), which are biodegradable, as substitutes for conventional petrochemical-based polymers. Developing better bacterial strains, better recovery and more efficient fermentation has been a goal.

South Korea, Statistical Data Included, Polymers, Fermentation

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