Resolution of chiasmata in oocytes requires separase-mediated proteolysis

Article Abstract:

A novel approach is described to study the role of mitotic proteins during meiosis I in mouse oocytes. A transgene is used to expresses Cre recombinase from the zona pellucida 3 promoter active during growing oocytes to delete exons that encode separase's protease domain from afloxed allele of the gene. The results have shown that proteolytic cleavage by separase is required for Rec8's removal from chromosome arms and for chiasma resolution but not for first polar body (PBE).

Analysis, Physiological aspects, Chromosome replication, Rats, Meiosis, Oocytes, Rattus

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A mitotic topoisomerase II checkpoint in budding yeast is required for genome stability but acts independently of Pds1/securin

Article Abstract:

A topoisomerase II (Topo II)-dependent G2/M checkpoint is defined in a genetically amenable eukaryote, budding yeast, and it is demonstrated that this checkpoint enhances cell survival. Neither DNA damage-responsive pathways nor Pds1/securin are needed for this checkpoint, and compromised Topo II function activates a yeast checkpoint system that operates by a novel mechanism.

United States, Genetic aspects, Eukaryotes, Mitosis, Yeast fungi, Yeasts (Fungi)

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Loss of the anaphase-promoting complex in quiescent cells causes unscheduled hepatocyte proliferation

Article Abstract:

The subunit Apc2 in mice is conditionally inactivated to investigate the role of anaphase-promoting complex or cyclosome (APC/C) in quiescent cells. It is demonstrated that the APC has an additional function to prevent hepatocytes from unscheduled re-entry into the cell cycle.

Cell cycle, Liver cells, Hepatocytes, Anaphase

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