Ribotyping to differentiate Fusobacterium necrophorum subsp. necrophorum and F. necrophorum subsp. funduliforme isolated from bovine ruminal contents and liver abscesses

Article Abstract:

Ribotyping Fusobacterium necrophorum cDNA hybridized with a probe transcribed from Escherichia coli rRNA is useful in detecting the genetic differences between Fusobacterium necrophorum subspecies. Isolates of the gram-negative bacteria from liver abscesses and ruminal contents of feedlot cattle are differentiated on the basis of distinctive bands in the hybridization patterns. The subsp. necrophorum which is virulent has a 2.6 kb band while, the subsp. funduliforme which is less virulent has a 4.3 kb band. The restriction endonuclease, EcoRI is the most effective ribotyping enzyme.

Observations, Gram-negative bacteria, Identification and classification

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Moderation of ruminal fermentation by ciliated protozoa in cattle fed a high-grain diet

Article Abstract:

Ciliated protozoans were found to have a moderating effect on the ruminal fermentation rate of steers fed a high-grain diet. Animals in the faunated group showed higher pH values and counts for lactate-fermenting bacteria, lower counts for anaerobic and lactate-producing bacteria and lower concentrations of fermentation products. This moderating effect appeared to result from the reduction in bacterial number and, possibly, reduction in bacterial activity.

Physiological aspects, Protozoa, Protozoans, Rumen fermentation

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Effect of forage or grain diets with or without monensin on ruminal persistence and fecal Escherichia coli O157:H7 in cattle

Article Abstract:

Twelve ruminally cannulated cattle, adapted to forage grain diet with or without monensin, were used to investigate the effects of diet and monensin on concentration and duration of ruminal persistence and fecal shedding of Escherchia coli 0157:H7.The results showed that cattle fed a forage diet were culture positive longer and with higher numbers than cattle on a grain diet.

Science & research, Health aspects, Risk factors, Escherichia coli infections

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