Role of pore size location in determining bacterial activity during predation by protozoa in soil

Article Abstract:

The bacterium Pseudomonas fluorescens is more likely to be killed by the soil ciliate Colpoda steinii but also more likely to be metabolically active if it is introduced into the soil through a medium- sized rather than a small pore. Researchers innoculated soil with both organisms. When the bacteria were introduced into the soil through a pore with a diameter of 6 micrometers to 30 micrometers the cell population decreased more rapidly than when they were introduced through a pore of less than 6 micrometers. However, the bacteria that survived in the larger pore were more active than those that survived in the small pore.

Observations, Predation (Biology), Pseudomonas fluorescens, Ciliata, Ciliates

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Molecular analysis of bacterial community structure and diversity in unimproved and improved upland grass pastures

Article Abstract:

The structure and diversity of bacterial communities in rhizospheres in improved and unimproved upland grass pastures, characterized by both plant species and fertilization regime, have been investigated. This was made possible by performing 16S ribosomal DNA sequence analysis of DNAs derived from triplicate soil plots. Results show significant differences in abundance in both replicate unfertilized, unimproved soil samples and fertilized, improved soil samples. No marked variations, however, were observed in the abundance of any phylogenetic sample.

Analysis, Biological diversity, Biodiversity, Grassland ecology, Grasslands, Rhizosphere, Pasture ecology, Pasture research

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Detection of a single genetically modified bacterial cell in soil by using charge coupled device-enhanced microscopy

Article Abstract:

Charge coupled device-enhanced microscopy was used to detect bioluminiscent bacteria in soil samples. Bioluminescence was induced by cloning luciferase genes from Vibrio harveyi into Pseudomonas syringae pv. phaseolicola. Despite being undetected by conventional methods, bioluminescent single cells were detected with ease in dark fields while being masked by soil particles. Potential applications of the technique are in tracking of genetically modified microbes in the soil environment.

Methods, Usage, Microbial genetic engineering, Charge coupled devices, CCDs (Charge coupled devices), Microscope and microscopy, Microscopes, Microscopy

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