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Role of spontaneous current oscillations during high-efficiency electrotransformation of thermophilic anaerobes

Article Abstract:

A pulse gated by a square signal generated by a custom generator is used to observe the current oscillations at about 24 MHz during electrotransformation (ET) of the thermophilic anaerobes Clostridium thermocellum ATCC 27405, C. thermocellum DSM 1313, and Thermoanaerobacterium saccharolyticum YS 485. The results have indicated that spontaneously arising oscillations have a large beneficial effect on transformation efficiency in the system employed here and that the development of oscillations in this system is affected by the cell species present.

Author: Lynd, Lee R., Tyurin, Michael V., Sullivan, Charles R.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
Bacteria, Thermophilic, Anaerobic bacteria, Frequencies of oscillating systems, Oscillating frequencies, Thermophiles

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Electrotransformation of Clostridium thermocellum

Article Abstract:

Clostridium thermocellum is an anaerobic endospore forming bacteria capable of fermenting cellulose and other beta-glucan substrates to ethanol and acetic acid. Electrotransformation of many strains of clostidium thermocellum was achieved by using plasmid pIkm1based on resistance to erythromycin and lincomycin.

Author: Lynd, Lee R., Tyurin, Michael V., Desai, Sunil G.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
Influence, Erythromycin, Anaerobiosis

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Kinetics and relative importance of phosphorolytic and hydrolytic cleavage of cellodextrins and cellobiose in cell extracts of Clostridium thermocellum

Article Abstract:

A detailed study of the kinetics of cellobiose phosphorylase(CbP) and cellodextrins phosphorylase(CdP) in Clostridium thermocellum cell extracts is presented. The results provide support that phosphorolytic cleavage of cellobiose and cellodextrins dominates hydrolytic cleavage in Clostridium thermocellum.

Author: Percival Zhang, Yi-Heng, Lynd, Lee R.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
Phosphorylase

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Subjects list: Research, Analysis, Clostridium
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