Role of spontaneous current oscillations during high-efficiency electrotransformation of thermophilic anaerobes

Article Abstract:

A pulse gated by a square signal generated by a custom generator is used to observe the current oscillations at about 24 MHz during electrotransformation (ET) of the thermophilic anaerobes Clostridium thermocellum ATCC 27405, C. thermocellum DSM 1313, and Thermoanaerobacterium saccharolyticum YS 485. The results have indicated that spontaneously arising oscillations have a large beneficial effect on transformation efficiency in the system employed here and that the development of oscillations in this system is affected by the cell species present.

Bacteria, Thermophilic, Anaerobic bacteria, Frequencies of oscillating systems, Oscillating frequencies, Thermophiles

---

Electrotransformation of Clostridium thermocellum

Article Abstract:

Clostridium thermocellum is an anaerobic endospore forming bacteria capable of fermenting cellulose and other beta-glucan substrates to ethanol and acetic acid. Electrotransformation of many strains of clostidium thermocellum was achieved by using plasmid pIkm1based on resistance to erythromycin and lincomycin.

Influence, Erythromycin, Anaerobiosis

---

Kinetics and relative importance of phosphorolytic and hydrolytic cleavage of cellodextrins and cellobiose in cell extracts of Clostridium thermocellum

Article Abstract:

A detailed study of the kinetics of cellobiose phosphorylase(CbP) and cellodextrins phosphorylase(CdP) in Clostridium thermocellum cell extracts is presented. The results provide support that phosphorolytic cleavage of cellobiose and cellodextrins dominates hydrolytic cleavage in Clostridium thermocellum.

Phosphorylase

Similar abstracts:

• Abstracts: Role of soil pH in the development of enhanced biodegradation of fenamiphos. A previously unexposed forest soil microbial community degrades high levels of the pollutant 2,4,6-trichlorophenol
• Abstracts: Relative ability of orally administered Lactobacillus murinus to predominate and persist in the porcine gastrointestinal tract
• Abstracts: Silencing of human polycomb target genes is associated with methylation of histone H3 Lys 27. A silencing pathway to induce H3-K9 and H4-K20 trimethylation at constitutive heterochromatin
• Abstracts: Assembly of an active translation initiation factor complex by a viral protein. Phosphorylation of eIF4E by Mnk-1 enhances HSV-1 translation and replication in quiscent cells.
• Abstracts: Aging in vertebrates, and the effect of caloric restriction: a mitochondrial free radical production-DNA damage mechanism?

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.