Role of tryptophan residues in toxicity of Cry1Ab toxin from Bacillus thuringiensis

Article Abstract:

A study was conducted to examine the role of Trp residues in the structure and function of Cry1Ab toxin, produced by Bacillus thuringiensis. The results show that W65 and W316 are important for insecticidal activity of the toxin since their replacement by Phe reduced toxicity against Manduca sexta.

Science & research, Genetic aspects, Bacillus thuringiensis, Diseases and pests, Tryptophan, Antibiotic residues

---

Production of chymotrypsin-resistant Bacillus thuringiensis Cry2Aa1 delta-endotoxin by protein engineering

Article Abstract:

Research was conducted to produce mutant forms of Cry2Aa1 delta-endotoxin of Bacillus thuringiensis using site-directed mutagenesis. Results show that midgut enzymes of gypsy moth on these mutant forms produce the active fragment that resists protease digestion.

United States, Methods, Usage, Proteases, Bacterial toxins, Control, Protein engineering, Mutagenesis, Gypsy moth, Gypsy moths

---

Similar abstracts:

• Abstracts: Role of hexose transport in control of glycolytic flux in Saccharomyces cerevisiae
• Abstracts: Microbial utilization of free and clay-bound insecticidal toxins from Bacillus thuringiensis and their retention of insecticidal activity after incubation with microbes
• Abstracts: Genotypes of cryptosporidium species infecting fur-bearing mammals differ from those of species infecting humans
• Abstracts: PCR assay of the groEL gene for detection and differentiation of Bacillus cereus group cells. part 2 Combined immunomagnetic separation-molecular beacon-reverse transcription-PCR assay for detection of hepatitis A virus from environmental samples
• Abstracts: High-level production of human leptin by fed-batch cultivation of recombinant Escherichia coli and its purification

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.