Use of repetitive DNA sequences and the PCR to differentiate Escherichia coli isolates from human and animal sources

Article Abstract:

Research has been conducted on the rep-PCR DNA fingerprint technique. The use of this technique in investigating the difference between human and animal fecal pollution sources is discussed.

Statistical Data Included, Analysis, Polymerase chain reaction, Feces, Microbiology, Microbiological research

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Sample size, library composition, and genotypic diversity among natural populations of Escherichia coli from different animals influence accuracy of determining sources of fecal pollution

Article Abstract:

A horizontal, flurophore-enhanced, repetitive extragenic palindromic-PCR (rep-PCR) DNA fingerprinting technique (HEFERP) was developed and evaluated as a means to differentiate human from animal sources of Escherichia coli. The results indicate that removal of duplicate genotypes within DNA fingerprint libraries, proper methods of statistical analysis and correct alignment of band data within and between gels improve the accuracy of microbial source tracking methods.

Pollutants produced & recycled, Methods, Composition, Waste management, Stool specimen analysis

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Substrate specificity and colorimetric assay for recombinant TrzN derived from Arthrobacter aurescens TC1

Article Abstract:

A study cloned the trzN gene from Arthrobacter aurescens TC1, expressed as His-tag protein in Escherichia coli, and purified to homogeneity. TrzN showed high activity with sulfur-substituted s-triazines and the highest activity with ametryn sulfoxide.

Science & research, Genetic aspects, Gene expression

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