Secretion of cryparin, a fungal hydrophobin

Article Abstract:

Secretion of cryparin, a fungal hydrophobin associated with the cell surface in the filamentous ascomycete Cryphonectria parasitica, is discussed based on a study to find out if biding of cryparin to cell walls precedes secretion into the culture fluid and to find out if it is glycosylated in the secretion process. Binding of the hydrophobin to the cell wall is different from that seen from other hydrophobins in that it goes first into the culture media in submerged liquid growth and rapidly associates after that with the mycelial cell wall. Evidence of secretion via vesicles through the cell wall and into the culture medium was found.

Cell membranes, Signal peptides, Plasma membranes, Molds (Fungi)

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Biofilms on indwelling urethral catheters produce quorum-sensing signal molecules in situ and in vitro

Article Abstract:

Research was conducted to examine the hypothesis that biofilms on indwelling urethral catheters produce quorum-sensing signal molecules in situ and in vitro. The Pseudomonas aeruginosa biofilms produced strongly positive reaction that were generated on catheters. Sections of the catheters from which biofilm had been taken also gave positive responses in the assay. Results along with negative reactions seen in unused catheters reveal that the quorum-sensing signal molecules may have been generated by the biofilm during its development in the catheter.

Gram-negative bacteria, Gene expression, Macrolide antibiotics, Macrolides, Pseudomonas aeruginosa, Urinary catheterization

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The bglA gene of Aspergillus kawachii encodes both extracellular and cell wall-bound beta-glucosidases

Article Abstract:

The gene encoding beta-glucosidase in Aspergillus kawachii has been cloned and its sequence analyzed. It was found that both of the extracellular beta-glucosidases and the cell wall-bound glucosidase are encoded by the gene, now designated bglA.

Japan, Genetic aspects, Aspergillus, Glucosidases, Alpha glucosidases

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