Selective enumeration of Bacteroides vulgatus and B. distasonis organisms in the predominant human fecal flora by using monoclonal antibodies

Article Abstract:

Monoclonal antibodies enumerate Bacteroides vulgatus and B. distasonis in feces of healthy individuals. The antibodies are specific and fail to react with other bacteria. Plates coated with diluted fecal samples are exposed to air to kill the oxygen-sensitive anaerobes. The total bacteria are counted and the concentration of Bacteroides cells is determined with monoclonal antibodies. B. vulgatus is present in all samples while B. distasonis is rarely present. The bacterial concentration is unaffected by small changes in the environment.

Usage, Measurement, Feces, Microbiology, Monoclonal antibodies

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Use of luciferase genes as biosensors to study bacterial physiology in the digestive tract

Article Abstract:

An experimental model was developed for the study of Lactococcus lactis promoter activities in the mouse digestive tract (DT). The physiology of the L. lactis was first determined using the luciferase gene, which served as a reporter gene. Results show that the model can have potential application for the study of promoter activity in bacterial cells found in the DT. It can also be applied in studies about microorganisms and their role as probiotic organisms.

Models, Physiological aspects, Bacterial genetics, Gene expression, Promoters (Genetics), Gastrointestinal system

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Differential activities of four Lactobacillus casei promoters during bacterial transit through the gastrointestinal tracts of human-microbiota-associated micefffffff

Article Abstract:

Luciferase mRNA in the gastrointestinal tract (GI) is quantified while investigating the activity of other Lactobacillus casei promoters in the GI tract in order to validate the results obtained from transcriptional fusion. The direct quantitative transcript analysis of fecal extracts from human-microbiota-associated (HMA) mice has confirmed that L. casei can initiate mRNA synthesis during transit through their GI tracts.

Science & research, Care and treatment, Messenger RNA, Gastrointestinal diseases, Luciferase

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