Sequence analysis of a 50 kb region between spoOh and rrnH on the Bacillus subtilis chromosome
Article Abstract:
The 49 630 bp region between the spoOH and rrnH gene of Bacillus subtilis was cloned and sequenced by polymerase chain reaction. The spoOH-rrnH region is composed of 61 open reading frames (ORFs) with high levels of ribonucleic acids (RNAs). The protein products of 51 ORFs have already been classified. The sequenced region is composed of four ribosomal RNAs and six transfer RNAs. Furthermore, five of the ORFs have ATG and GTG initiation codons while four ORFs have TTG initiation codons.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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New genes in the 170 degrees region of the Bacillus subtilis genome encode DNA gyrase subunits, a thioredoxin, a xynalase and an amino acid transporter
Article Abstract:
The 26.2 kb fragment located in the 170 degrees region of Bacillus strain deoxyribonucleic acid genome was sequenced and cloned by the amplification of selected restriction sites of specific genes. DNA sequencing of the 26.2 kb fragment indicated the presence of aconitase (citB) genes and transketolase (tkt) genes in the 26 open reading frames of the 170 degrees region. The DNA fragment also contains grlA and grlB DNA gyrase A and B genes.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Sequence and analysis of a 31 kb segment of the Bacillus subtilis chromosome in the area of the rrnH and rrnG operans
Article Abstract:
Genetic research has established a 31141 bp nucleotide sequence in the genome of organism Bacillus subtilis, strain 168, from trnl to pNEXT52. Five transfer ribosomal nucleic acid genes, two ribosomal ribonucleic acid operons and 22 ORFs were identified. Strain 168 contains the gene cluster feuA, B, and C.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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