Stabilization of stalled DNA replication forks by the BRCA2 breast cancer susceptibility protein

Article Abstract:

It is demonstrated that by using two-dimensional gel electrophoresis, y-shaped DNA junctions at stalled replication forks disappear during genome-wide replication arrest in BRCA2-deficient cells, accompanied by double-strand DNA breakage. It is proposed that in BRCA2 deficiency and related chromosomal instability diseases, the breakdown of replication forks, which arrest or pause during normal cell growth, triggers spontaneous DNA breakage, leading to mutability and cancer predisposition.

Care and treatment, Causes of, Breast cancer, DNA replication

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Top1- and Top2- mediated topological transitions at the replication forks ensure fork progression and stability and prevent DNA damage checkpoint activation

Article Abstract:

The article describes that replicating chromatin Top-1 and Top-2 act within a 600-base 'pair (bp) region spanning the moving forks. The failure in resolving fork-related topological constrains during S-phase may result in abnormal chromosome transitions, DNA damage checkpoint activation, and chromosome breakage during segregation.

DNA damage, Chromosome replication

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Replication of mitochondrial DNA occurs by strand displacement with alternative light-strand origins, not via a strand-coupled mechanism

Article Abstract:

Atomic force microscopy and 2D agarose gel electrophoresis are used for examining mitochondrial DNA (mtDNA) replicative intermediates from mouse liver. It is observed that strand-displacement replication is the dominant mode of mtDNA replication in mouse liver.

Science & research, Research, Mitochondrial DNA, Atomic force microscopy

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