Synthesis of novel lipids in saccharomyces cerevisiae by heterologous expression of an unspecific bacterial acyltransferase

Article Abstract:

The bifunctional wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase (WS/DGAT) is the key function in storage lipid accumulation in the gram-negative bacterium Acinetobacter calcoaceticus ADP1. The findings confirm that WS/DGAT is capable of utilizing a broad range of alcohols as substrates comprising long-chain fatty alcohols like hexadecanol as well as short-chain alcohols like ethanol.

Science & research, Research, Lipids

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Investigation of two distinct flavone synthases for plant-specific flavone biosynthesis in Saccharomyces cerevisiae

Article Abstract:

The biosynthesis of flavones by two distinct flavone synthases is investigated by introducing a soluble flavone synthase I (FSI) and a membrane-bound flavone synthase II (FSII) into the flavanone-producing recombinant yeast strain. The results have shown that unlike luteolin, the 5,7,4'-trihydroxyflavone apigenin inhibits flavanone biosynthesis in vivo in a nonlinear, dose-dependent manner.

Methods, Genetic aspects, Biosynthesis, Flavonoids, Flavones, Chemical properties, Bioflavonoids

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