The 1-kb-repeat-encoded DNA-binding protein as repressor of an alpha-glucosidase operon flanking the amplifiable sequence AUD1 of Streptomyces lividans

Article Abstract:

Results demonstrate that the proteins encoded by the 1-kb-repeat exhibit dual functions in that they repress agl genes and promote AUD1 element amplification in Streptomyces lividans.

Statistical Data Included, Gene mutations, Gene mutation, Glucosidases, Alpha glucosidases

---

An amplifiable and deletable locus of Streptomyces RP181110 contains a very large gene homologous to polyketide synthase genes

Article Abstract:

Molecular techniques revealed that production of the macrolide antibiotic spiramycin by Streptomyces ambofaciens RP181110 is governed by an ORF (orfPS) in the AUD205 locus. Genomic rearrangements resulted in the loss of spiromycin production but amplification of the AUD205 locus restored spiromycin biosynthesis. Sequencing of the orfPS gene revealed four sequences including a reiterated sequence corresponding to the acyltransferase domain of the protein. Since amplification caused overtranscription of the gene, it was suggested that orfPS encodes a polyketide synthase that affects spiramycin biosynthesis.

Macrolide antibiotics, Macrolides, Genetic transcription, Transcription (Genetics)

---

AUD4, a new amplifiable element from Streptomyces lividans

Article Abstract:

This paper describes identification and mapping of a 8.2 kilobase DNA sequence in a transformed strain of Streptomyces lividans. Results show that the sequence is an amplifiable unit of DNA belonging to class I amplifiable sequence and is located in the Asel-D fragment of the bacterial chromosome.

Physiological aspects, Nucleotide sequence, Base sequence, Bacterial genetics, Chromosome deletion

Similar abstracts:

• Abstracts: Accumulation of the angucycline antibiotic rabelomycin after disruption of an oxygenase gene in the jadomycin B biosynthetic gene cluster of Streptomyces venezuelae
• Abstracts: Bacterial fibronectin-binding proteins and endothelial cell surface fibronectin mediate adherence of Staphylococcus aureus to resting human endothelial cells
• Abstracts: Linkage of the gene for uncoupling protein to esterase-1,2 and haptoglobin in the rat. Mapping of a QTL for serum HDL cholesterol in the rabbit using AFLP technology
• Abstracts: The coronin-like protein POD-1 is required for anterior-posterior axis formation and cellular architecture in the nematode Caenorhabditis elegans

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.