Cytochrome c(sub)3 mutants of Desulfovibrio desulfuricans

Article Abstract:

Results show that the tetraheme cytochrome c(sub)3 plays a role in the electron transport pathway in Desulfovibrio desulfuricans bacteria.

United States, France, Statistical Data Included, Analysis, Physiological aspects, Gene mutations, Gene mutation, Genetic aspects, Electron transport, Sulfur bacteria

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Transposon mutagenesis in Desulfovibrio desulfuricans: development of a random mutagenesis tool from Tn7

Article Abstract:

Transposon mutagenesis is a new molecular tool that is increasingly being used in bacterial genetics research. In a study of the sulfate-reducing bacterium Desulfovibrio desulfuricans G20, site-specific insertion of transposon Tn7 into sites identified in other gram-negative bacteria created a mutation in the tnsD gene. Inactivation of this gene resulted in the transposition of a derivative of Tn7. Other transposons which exhibited this activity were the derivatives of Tn5 but not wild-type Tn5.

Methods, Transposons, Mutagenesis

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The Desulfuromonas acetoxidans triheme cytochrome c7 produced in Desulfovibrio desulfuricans retains its metal reductase activity

Article Abstract:

Research was conducted to examine the cloning of the gene encoding Desulfuromonas acetoxidans cytochrome c7. A new expression vector based on the sequence of the cyc gene encoding D vulgaris Hildenborough cytochrome c3 was constructed which permitted the heterologous production of holocytochrome c7 in D desulfuricans. Results indicate that D desulfuricans affords functional multiheme c-type cytochromes from bacteria belonging to a different genus and can be considered a suitable host for the heterologous biogenesis of multiheme c-type cytochromes.

Proteins, Protein synthesis, Genetic vectors

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