The chromosomes of Citrus and Poncirus species and hybrids: identification of characteristic chromosomes and physical mapping of rDNA loci using in situ hybridization and fluorochrome banding

Article Abstract:

Recombinant DNA (rDNA) sites on root-tip metaphase chromosomes of Citrus sinensis, Poncirus trifoliata and hybrids were located through in situ hybridization of 18S-5.8S-25S rDNA probes labeled with biotin or rhodamine and 5S rDNA probes labeled with digoxigenin. P. trifoliata had seven 18S-25S sites while C. sinensis had five and three different hybrids had five or six sites. Four 5S rDNA sites were observed which were mostly linked to 18S-25S rDNA sites. Results indicated high levels of chromosomal heterozygosity in all accessions studied.

Citrus, Recombinant DNA, Plant chromosomes

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Behavior of parental genomes in the hybrid Hordeum vulgare x H. bulbosum

Article Abstract:

Improved in situ hybridization methods were used to analyze the parental origin and movement of chromosomes in the hybrid between Hordeum vulgare and H. bulbosum. The parental genomes were found to be spatially separated throughout the cell cycle with the chromosomes of H. vulgare origin in a more central position than those of H. bulbosum origin. Movement of the cells through anaphase, telophase and interphase were also described.

Cell cycle, Barley

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Karyotype of slash pine (Pinus elliottii var. elliottii) using patterns of fluorescence in situ hybridization and fluorochrome banding

Article Abstract:

An accurate karyotype of the slash pine species is necessary to accurately create homologous group designations and determine valuable hybrids. Fluorescent in situ hybridization was used to identify the slash pine chromosomes through fluorochrome banding and morphology. DNA mapping can be used to identify slash pines and other varieties of conifers.

Usage, Fluorescence microscopy, Karyotypes, Pine, Pines

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