The general secretion pathway of Erwinia carotovora subsp. carotovora: analysis of the membrane topology of OutC and OutF

Article Abstract:

The membrane topology of the Out proteins of out genes in Erwinia carotovora subspecies carotovora (ECC) was analyzed to determine genetic mechanisms of the type II secretion system or general secretory pathway (GSP). Analysis of out gene membrane topology in ECC out gene-blaM fusions indicated the presence of highly homologous Out proteins that are encoded by the ECC out gene cluster. Furthermore, the secretion of the periplasmic enzyme for GTP was dependent on the interaction of OutF, OutL and OutE.

Genetic aspects, Microbial metabolism, Microbial enzymes, Bacteria, Phytopathogenic, Phytopathogenic bacteria

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Erwinia carotovora DsbA mutants: evidence for a periplasmic-stress signal transduction system affecting transcription of genes encoding secreted proteins

Article Abstract:

A study conducted on dsbA genes isolated from Erwinia carotovora subsp. carotovora (Ecc) and Erwinia carotovora subsp. atroseptica (Eca), including dsbC genes from Ecc, revealed the severe effect of pectate lyase activity and secretion on the Ecc mutants. The results, which also showed all dsb mutants with reduced tissue maceration in planta, indicates the presence of a feedback regulation mechanism where periplasmic disulfide bond formation defects act as a regulating signal for gene expression.

Gene mutations, Gene mutation, Plant genetics, Gene expression, Mutagenesis

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Cryptic carbapenem antibiotic production genes are widespread in Erwinia carotovora: facile trans activation by the carR transcriptional regulator

Article Abstract:

A study was conducted to analyze the distribution of carbapenem production genes in Erwinia carotovora strains. A dideoxynucleotide chain-termination technique was utilized to perform DNA sequencing. Overnight culture of Erwinia sample strains were harvested onto an agar lawn of Escherichia coli strain ESS. Results indicated that a substantial part of the cryptic genes resulted from defects in the carbapenem-specific, pheromone-depdent, CarR regulator.

DNA, Escherichia coli, Pathogenic microorganisms

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