The relationship between external glucose concentration and cAMP levels inside Esccherichia coli: implications for models of phosphotransferase-mediated regulation of adenylate cyclase

Article Abstract:

cAMP assay and measurement of expression of cAMP-regulated genes were conducted to determine the relationship between external glucose concentration and cAMP levels inside Escherichi coli. Results reveal that E. coli controls its cAMP levels through two or more transitions influenced by external glucose concentration and does not respond to decreasing saturation of glucose transport. Furthermore, results present implications for models of phosphotransferase-mediated regulation of adenylate cyclase.

Cyclic adenylic acid, Cyclic adenosine monophosphate, Glucose

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Filling the gap between hns and adhE in Escherichia coli K12

Article Abstract:

The Escherichia coli K12 genome has a 3060-nucleotide sequence between the hns and adhE genes. The sequence does not completely cover the space between the genes, but does fill most of it. The nucleotide sequence was discovered by subcloning a fragment that complemented a defective hns and evaluating it for thymidine kinease activity, and finally sequencing it. The region has 12 features, six of which direct and six of which complement the sequence.

Genomes, Insertion elements, DNA, DNA insertion elements

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Regulation of Escherichia coli adenylate cyclase activity during hexose phosphate transport

Article Abstract:

Previous research has shown low cAMP levels in Escherichia coli grown on glucose 6-phosphate (Glc6P). To explain this phenomenon, the mechanism leading to the low cAMP level when Glc6P is the carbon source is investigated. Results indicate that Flc6P is not directly responsible for the low cAMP level in E. coli but rather that the level is determined by a flux in the Glc6P through the UhpT.

Biological transport

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