The nuclear ribosomal DNA intergenic spacer as a target sequence to study intraspecific diversity of the ectomycorrhizal basidiomycete Hebeloma cylindrosporum directly on Pinus root systems

Article Abstract:

The amount and distribution of nuclear ribosomal DNA intergenic spacer (IGS) polymorphisms in populations of Hebeloma cylindrosporum found along the Atlantic coast of France have been studied. H. cylindrosporum is one of the dominant ectomycorrhizal fungi species associated with Pinus pinaster growing on the backs of the coastal sand dunes. Polymerase chain reactions were used in amplifying the IGS sequence from 125 haploid homokaryotic strains collected in 14 P. pinaster stands. Results indicated that the IGS is highly variable even within local populations and two restriction enzymes are only needed to identify the various allelic forms.

Analysis, DNA, Fungi, Diseases and pests, Pine, Pines, Mycorrhizas, Mycorrhizae

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Simple colorimetric method for detecting degenerate strains of the cultivated basidiomycete Flammulina velutipes (Enokitake)

Article Abstract:

A simple colorimetric method to detect degenerate strains of Flammulina velutipes by using a liquid medium supplemented with bromothymol blue and lactose was developed. The ability of the Flammulina velutipes strain to develop normal mushrooms could be determined by the color of the medium.

Science & research, Research, Mushrooms, Colorimetric analysis, Basidiomycota, Colourimetric analysis

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marY1, a member of the gypsy group of long terminal repeat retroelements from the ectomycorrhizal basidiomycete Tricholoma matsutake

Article Abstract:

Researchers describe a retroelement from Tricholoma matsutake that can be used for phylogenetic analysis of ectomycorrhizal basidiomycetes. Retroelements are retrovirus-like nucleic acids that occur in many eukaryotic genomes.

Edible mushrooms, Mushrooms, Edible

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