Ti plasmids from Agrobacterium characterize rootstock clones that initiated a spread of crown gall disease in Mediterranean countries

Article Abstract:

Research was conducted to examine the integrated physiological and molecular analyses of 30 Agrobacterium isolates derived from crown gall tumors and of various reference strains. The determination of the biovar, analysis of 16S ribosomal DNA restriction fragment length polymorphisms by PCR (PCR-RFLP), elucidation of the opine tupe and PCR-RFLP analysis of genes involved in virulence and oncogenesis were used in the characterization of the plasmids. Results provide strong evidence that the causal agent of crow gall disease in rose plants is transmitted via rootstock material.

Causes of, Plasmids, Titanium, Agrobacterium tumefaciens, Crown-gall disease, Crown gall disease

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New genetic techniques for group B streptococci: high-efficiency transformation, maintenance of temperature-sensitive pWV01 plasmids, and mutagenesis with Tn917

Article Abstract:

Microbiological research has established that the genomic mutant Tn917 transposes to the Group B Streptococcus agalactiae chromosome at a frequency of ten (super -3) CFU. Three new genetic manipulation methods have been devised using the temperature-sensitive pWV01 plasmid pTV10K as a transposon delivery vector to assess the virulent characteristics of the pathogenic microorganism. Further research into cloning and mutagenesis can be carried out using the techniques.

Methods, Streptococcal infections, Genetic research, Streptococcus agalactiae

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Development of a nonantibiotic dominant marker for positively selecting expression plasmids in multivalent Salmonella vaccines

Article Abstract:

Researchers used the bar gene as a marker for selecting expression plasmids in multivalent Salmonella vaccines. This gene confers resistance to the herbicide DL-phosphinothricin. It eliminates the problem of using plasmids that have antibiotic resistance genes.

Prevention, Salmonellosis, Bacterial vaccines

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